How to Properly Mix Peptide PT-141 for Research Purposes



PT-141, a synthetic peptide, typically arrives in a lyophilised (freeze-dried) form, which preserves the peptide for extended storage. Reconstituting PT-141 with accuracy is crucial to maintaining its bioactivity and ensuring accurate measurements for research applications.

This guide will outline the steps needed to properly mix PT-141, ensuring it is prepared accurately and efficiently for research purposes.

Required Materials

Before starting the mixing process, ensure you have the following materials:

  • PT-141 Peptide Vial (10mg)
  • Bacteriostatic Water (2ml)
  • Alcohol Swabs
  • 1ml Mixing Syringe (30g/29g recommended)
  • Sterile Gloves

Note: 10mg of PT-141 equals 10,000mcg. We will mix it with 2ml of bacteriostatic water, and each 10 units (iu) on an insulin syringe will equate to 500mcg of PT-141.

Initial Preparations

Follow these steps to ensure proper handling:

  1. Clean your hands thoroughly: Wash with soap and water to avoid contamination.
  2. Wear sterile gloves: Gloves will provide extra protection and prevent contamination.

Step-by-Step Guide to Mixing PT-141

1. Allow the PT-141 to Reach Room Temperature

If the peptide has been stored in a freezer or medical fridge, let it reach room temperature before reconstitution. The same applies to the bacteriostatic water.

2. Open the Vials

Carefully remove the caps from both the PT-141 vial and the bacteriostatic water vial. Use an alcohol swab to clean the tops of both vials.

3. Equalise the Pressure in the Peptide Vial

To avoid spraying when adding bacteriostatic water, equalise the pressure:

  1. Draw half a syringe (0.5ml) of air.
  2. Insert the syringe into the PT-141 vial and release the air to balance the internal pressure.

4. Add the Mixing Solution

Using the syringe:

  1. Withdraw 1 ml of bacteriostatic water.
  2. Insert the syringe at a 45-degree angle into the PT-141 vial, allowing the liquid to flow down the side of the vial. This prevents foam or bubbles from forming.
  3. Repeat the process to add another 1 ml of bacteriostatic water (for a total of 2 ml).

5. Gently Mix the Solution

Gently swirl the vial between your fingers to dissolve the peptide into the solution. Avoid shaking the vial as this can damage the peptide structure.

Measuring the Research Dose

With PT-141 mixed in 2ml of bacteriostatic water:

  • Each 10 units (iu) on a mixing syringe equals 500mcg of PT-141.
  • To measure 1,000mcg, withdraw 20 units (0.2ml) from the vial.

Post-Mixing Care

To maintain the integrity of the reconstituted peptide:

  • Store the vial in a refrigerator at 2-8°C. Do not freeze it again after reconstitution.
  • Use within 30 days to ensure optimal results.

Final Notes

By following this guide, you can ensure that PT-141 is reconstituted properly for research applications. Accurate mixing is crucial for maintaining peptide bioactivity and ensuring reliable results in research settings.

For more information, visit our website at UK Peptides or contact us for further assistance and support with your peptide needs.